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Brain Observatory Labview Planning

drivers ¶

• drivers page
• Microscope
• ludl (dll's were in the directory, i think)
• microfire camera
• image aquisition software
  • NI Labview Machine vision software
    

• Microscope startup kit
  

Rough development outline ¶

• connect insturmenation
• capture single image
• move in z axis, capture images
• move in single X or y axis, capture
• move in small grid, capture images
• capture microscope metadata
• for each image capture microscope metadata
  

Tests ¶

• capture microscope metadata
• can capture a series of images
• can capture a multiple series of images (aka different magnifications)
  

Imaging thoughts ¶

1. need (x,y,z) stepsize for different scales
   • calculate expected microns
2. need optical correction
3. background correction image
4. what is needed to capture image
   • check to see that filter is the correct one
   • open shutter
   • speed
5. camera/imaage capture link should not adjust/stretch image
   

Information to capture ¶

• Microscope metadata
• image series
  • what should be out file naming convention?
    

Labview examples/papers ¶

• Microscope stage controller using LabVIEW
• A Control and Acquisition System for a Two-Photon Fluorescence Microscope
• Telepathology Microscope Control to Medical Clinics Via ...Internet
• Project MAEROL: Microscope Access Everywhere Remote Optics Laboratory
• http://www.lrz-muenchen.de/~chr/2Photon-setup.html
  

• Image acquisition and processing with LabVIEW
  

• Image Acquisition
• search for autofocus
• image correlation
• timestamping image
• magnafire camera
• Adjusting the camera parameters